PHI

Description

Adaptive optics through the camera port

• Plug-and-playAttaches to any standard C-mount camera port. Connect power and USB, and you are ready to go.
• ModularEasily insert or remove the DPP to switch between standard imaging and adaptive optics.
• Adaptive opticsCorrect up to the 7th radial order of Zernike modes. Automatically optimize image quality for thick, scattering tissue samples.
• Motionless 3D focusingIntegrated tunable lens at the conjugate pupil plane with up to 16 diopters of focusing range: a z-stack costs no stage motion, no settling time and no mechanical disturbance of the sample.
• Future-proofWorks with current and upcoming DPPs: swap in the one that suits your application, without replacing the platform.
• Stays on the portBetween experiments PHI runs in bypass at > 90 % average transmission, 450 to 1050 nm — it earns its place on the camera port whether or not you are correcting that day.

 

 

 

 

See what PHI does

Results from partner labs: aberration the sample introduces, deliberate wavefront shaping, and single-molecule localization.

Correcting what the sample introduces

a wrong immersion medium — on a specimen, and on point sources — Life Imaging Center, University of Freiburg

 

 

 

 

 

 

 

Single-molecule localization

the run and the PSF underneath it — TU Wien, Biophysics Research Unit

 

 

 

 

 

 

 

 

                                    Without AO                                                                                                      With AO

 

 

Mobile ATTO488-DOPE in a supported lipid bilayer under TIRF illumination, ZEISS Plan-Apochromat 100×/1.46. PHI corrects the sample-induced aberration while the localization run is acquiring.TU Wien, Institute of Applied Physics, Biophysics Research Unit. Courtesy of Prof. Gerhard Schütz and Dr. Mario Brameshuber.

   

 

 

 

 

 

 

 

 

 

                                                         Aberrated.                                                                        Corrected

The measured 2D point-spread function from the same single-molecule setup, both panels on the same scale. Localization accuracy depends on PSF symmetry: the aberrated PSF is elongated and tilted, the corrected one is round.TU Wien, Institute of Applied Physics, Biophysics Research Unit. Courtesy of Prof. Gerhard Schütz and Dr. Mario Brameshuber.

 

Thick tissue

the hardest case — aberration the sample itself introduces, varying across the field

 

Specifications

PHI at a glance

What it attaches to and what the camera port has to carry, what it does to the wavefront, what it needs to run — and, at the end, whether your own objective’s pupil lands inside the window.

Compatibility

Microscope	Mounts onto any standard C-mount port of upright or inverted microscope frames Chain multiple C-mount devices: confocal & spinning-disk modules, structured illumination, etc.
Objective lens	Covers objectives with pupil diameters from 5 mm to 20 mm 5–11 mm using the Delta 7-10 · 11–20 mm using the Delta 7-20
Camera	C-mount cameras supported F-mount version in preparation
Software	µManager, ScanImage, ThorImage, ZEN blue or the Python SDK Nikon NIS-Elements integration in preparation
Overall envelope (W × D × H)	367.5 × 230.4 × 132 mm
Camera port (in)	C-mount female (internal thread)
Camera port (out)	C-mount male (external thread)
Weight	8.3 kg

Performance

Adaptive optics

• Maximum peak-to-valley of the corrected wavefronts: > 10 µm
• Maximum spatial frequency of the correction: 7th radial order of Zernike modes
• Response time < 40 ms

Remote focusing

• Tuning range up to 16 dpt
• Tuning precision 0.001 dpt
• Response time 3 ms

Optical transmission

• AO & remote-focusing mode ~ 75 % average, 450–1050 nm
• Bypass mode > 90 % average, 450–1050 nm

General

• Power & connectivity 12 V wall power, USB to PC
• Main applications spherical-aberration correction, deep-tissue imaging, remote focusing, 3D imaging

Mechanical envelope

The DPP exchange slot on the side panel and the insertable pinhole on the output side stay reachable while the unit is mounted: swap the Delta 7 without taking PHI off the microscope.

Camera release lever · DPP & camera adjustment nubs.

All dimensions in millimeters.

Objective lens

Mag	4×	10×	20×	25×	40×	63×	100×
Min NA	0.2compatible	0.25compatible	0.4compatible	0.7compatible	0.8compatible	1.2compatible	1.25significantly reduced performance
Max NA	0.28significantly reduced performance	0.45compatible	0.75compatible	1.1compatible	1.3compatible	1.45compatible	1.45compatible

CompatibleSignificantly reduced performance

Typical NA range each magnification is sold in, against PHI’s 5–20 mm pupil window at a 200 mm tube lens; Olympus (180 mm) and ZEISS (165 mm) frames shift the result.

D_pupil ≃ 2 · (f_tube / M) · NAf_tube tube-lens focal length: 200 mm on most frames, 180 mm Olympus, 165 mm ZEISS · M magnification · NA numerical aperture